Difference between revisions of "User:Daniel Goodman/Notebook/Cluzel/2010/03/03"

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(Glycerol Stocks for MG1655 strains)
(Glycerol Stocks for MG1655 strains)
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Forgot to take out the cultures on Friday, so doing this again today.
 
Forgot to take out the cultures on Friday, so doing this again today.
  
<span color=red> Reminder: Take culture tubes out of shaker at ~6:30 and put stocks in glycerol!</span>
+
<span style="color: red"> Reminder: Take culture tubes out of shaker at ~6:30 and put stocks in glycerol!</span>
  
 
==Agarose==
 
==Agarose==

Revision as of 09:54, 3 March 2010

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Cluzel Lab Notebook
Daniel Goodman
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Glycerol Stocks for MG1655 strains

  • 2 culture tubes
  • 4 ml LB in each
  • 4 ul of Amp in one (for GFP strain)
  • took one average-sized colony from each
  • put in 37°C shaker (at ~12:30 pm)

Forgot to take out the cultures on Friday, so doing this again today.

Reminder: Take culture tubes out of shaker at ~6:30 and put stocks in glycerol!

Agarose

  • making 5% agarose (.3 g in 6 mL) for molds that are easier to manipulate and less likely to fracture/distort