Difference between revisions of "User:Daniel Goodman/Notebook/Cluzel/2010/03/03"

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(Agarose)
(Glycerol Stocks for MG1655 strains)
Line 5: Line 5:
 
* 4 ul of Amp in one (for GFP strain)
 
* 4 ul of Amp in one (for GFP strain)
 
* took one average-sized colony from each
 
* took one average-sized colony from each
* put in 37°C shaker (at 12:15 pm)
+
* put in 37°C shaker (at ~12:30 pm)
  
 
Forgot to take out the cultures on Friday, so doing this again today.
 
Forgot to take out the cultures on Friday, so doing this again today.
 +
 +
{{red||Reminder: Take culture tubes out of shaker at ~6:30 and put stocks in glycerol!}}
  
 
==Agarose==
 
==Agarose==

Revision as of 10:53, 3 March 2010

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Cluzel Lab Notebook
Daniel Goodman
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Glycerol Stocks for MG1655 strains

  • 2 culture tubes
  • 4 ml LB in each
  • 4 ul of Amp in one (for GFP strain)
  • took one average-sized colony from each
  • put in 37°C shaker (at ~12:30 pm)

Forgot to take out the cultures on Friday, so doing this again today.

Agarose

  • making 5% agarose (.3 g in 6 mL) for molds that are easier to manipulate and less likely to fracture/distort