Difference between revisions of "User:Daniel-Mario Larco/Notebook/AU Biodesign Lab - 09/03/2013/2014/04/22"

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(Autocreate 2014/04/22 Entry for User:Daniel-Mario_Larco/Notebook/AU_Biodesign_Lab_-_09/03/2013)
 
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==Entry title==
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==Objective==
* Insert content here...
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1. Testing Pellets and Supernatants of concentrated solutions
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Notes
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Thursday 04/17, I ran the solutions in the centrifuge for 3 hours at 18,000 rpm
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The pellet was much larger than before. the supernatant was removed and store for testing
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==Procedure==
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#The solutions were placed in the centrifuge for a second spin down for 3 hours at 1,8000 rpm
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#Tris buffer and Sodium DIthionite in Tris buffer were degassed
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#Ocean Optics spectroscopy of pellets and supernatants (1st and 2nd)
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==Data==
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[[Image:Alpha Hemoglobin Pellet.png+DML|300px]]
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The results for the hemoglobin pellet after correction
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[[Image:Alpha Myoglobin Pellet.png+DML|300px]]
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The results for the myoglobin pellet after correction
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[[Image:AuNPDTT.png|300px]]
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AuNP solution run with Sodium Dithionite to correct protein solutions
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Revision as of 01:44, 25 April 2014

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Objective

1. Testing Pellets and Supernatants of concentrated solutions

Notes Thursday 04/17, I ran the solutions in the centrifuge for 3 hours at 18,000 rpm The pellet was much larger than before. the supernatant was removed and store for testing

Procedure

  1. The solutions were placed in the centrifuge for a second spin down for 3 hours at 1,8000 rpm
  2. Tris buffer and Sodium DIthionite in Tris buffer were degassed
  3. Ocean Optics spectroscopy of pellets and supernatants (1st and 2nd)

Data

Alpha Hemoglobin Pellet.png+DML The results for the hemoglobin pellet after correction

Alpha Myoglobin Pellet.png+DML The results for the myoglobin pellet after correction

AuNPDTT.png AuNP solution run with Sodium Dithionite to correct protein solutions