User:Daniel-Mario Larco/Notebook/AU Biodesign Lab - 09/03/2013/2014/04/16: Difference between revisions
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== | ==Objective== | ||
1. Test Spun down proteins for activity | |||
2. Make conjugates at a much higher concentration | |||
==Procedure== | |||
-Testing Myoglobin and Hemoglobin | |||
#Use Ocean optics | |||
##Sample of centrifuged solution | |||
##10mM Degassed Sodium Dithionite | |||
-Making new conjugates for the alpha method | |||
#25mL of AuNP solution | |||
#25mL of protein (myoglobin and hemoglobin) in 50mM Tris buffer | |||
#Placed in fridge until testing | |||
==Data== | |||
[[Image:MyoglobinDTT.png+DML|300px]] | |||
This is the result of the centrifuged Myoglobin solution | |||
It shows Myoglobin activity which means spinning at high speeds does not denature protein or break them apart | |||
The same result was found for the Hemoglobin solution | |||
Revision as of 02:34, 25 April 2014
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Objective1. Test Spun down proteins for activity 2. Make conjugates at a much higher concentration Procedure-Testing Myoglobin and Hemoglobin
-Making new conjugates for the alpha method
Data
The same result was found for the Hemoglobin solution
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