User:Daniel-Mario Larco/Notebook/AU Biodesign Lab - 09/03/2013/2013/09/25
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To run SDS-PAGE to observe amount of hemoglobin remaining after yesterday's digestion. This should be a complimentary measurement to our UV-Vis analysis from yesterday.
We will be using a Bio-Rad Mini Protean system with pre-cast Mini Protean TGX gels. The manual for this system can be found here We will be running SDS-PAGE followed by gel development with Coomassie Blue staining.
Below is a short description of how we will proceed. Please refer to the manual for more detailed instructions
From left to right on the image, we have wells 1-12.
-Well 1: Hemoglobin
-Well 2: Pepsin 0.5 hr
-Well 3: Pepsatin 0.5 hr
-Well 4: Pepsin 1 hr
-Well 5: Pepsatin 1hr
-Well 6: Pepsin 1.5 hr
-Well 7: Pepsatin 1.5 hr
-Well 8: Pepsin 2 hr
-Well 9: Pepsatin 2 hr
-Well 10: Pepsin overnight
-Well 11: Pepsatin overnight
-Well 12: Hemoglobin