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Sean Clarke

saclarke at MIT

I am a first-year Biological Engineering (BE, course 20) graduate student who worked in the Endy Lab this past summer 2005. This semester I am taking classes, and looking for a lab I would like to join. The Endy lab is one possibility. I naively expected to be able to continue some lab work, but classes and other demands of the first year have made that practically impossible.

My background is in mechanical engineering and design, but not of things quite as small as BioBricks.

Potential research topics

  • Scaling of engineered biology
  • Replicating biological machines
  • Technologies/vocabularies to make biological engineering easier
  • Biomimicry, bioscaffolds for material processing
  • Recycling/"cradle to cradle" design of biological systems
  • Usability of biological design software or methods



  • My first project was the Orthogonal cloning of clpXP from E. coli into yeast. So far I've been more successful at learning molecular biology and techniques by making mistakes than at cloning into yeast. My work is documented in a lab notebook, but I would like to move to a wiki or other on-line lab notebook for searching, organization, and linking.
  • Another useful project would be the construction of a low copy number plasmid for use with BioBricks. It might be based on the F or mini-F plasmid from E. coli.