Difference between revisions of "User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/27"

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==Entry title==
 
  
  
  
==Objective==
 
*Finish Kinetic Data and calculate average
 
*Begin UV runs with calculated inhibitor
 
  
==Description==
 
*Procedure from yesterday was continued with the same concentrations. Yesterday two trials were completed. The third was completed today.
 
**The following concentrations were used.
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''Concentration [Ado] (uM)'''
 
| align="center" style="background:#f0f0f0;"|'''Velocity (nm/sec)'''
 
| align="center" style="background:#f0f0f0;"|'''1/[Ado] (1/uM)'''
 
| align="center" style="background:#f0f0f0;"|'''1/velocity (sec/nm)'''
 
| align="center" style="background:#f0f0f0;"|''''''
 
| align="center" style="background:#f0f0f0;"|'''(-1)/Km'''
 
| align="center" style="background:#f0f0f0;"|'''-0.022385428'''
 
|-
 
| 10.52||3.70166E-05||0.095057034||27014.92537||||Km||44.67191792
 
|-
 
| 12.34||5.13812E-05||0.081037277||19462.36559||||1/Vmax||2662.131337
 
|-
 
| 14.93||6.0221E-05||0.066979236||16605.50459||||Vmax||0.000375639
 
|-
 
| 18.87||7.45856E-05||0.052994171||13407.40741||||||
 
|-
 
| 25.64||8.67403E-05||0.03900156||11528.66242||||||
 
|-
 
| 40||9.94475E-05||0.025||10055.55556||||||
 
|-
 
|
 
|}
 
  
*10mM Quercetin stock was prepared.
 
*Cuvettes with the following solutions were prepared and analyzed.
 
**Today, we only had time to complete the first two.
 
  
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''[ADA Stock Solution] (U/mL)'''
 
| align="center" style="background:#f0f0f0;"|'''Volume ADA Stock Solution Added (mL)'''
 
| align="center" style="background:#f0f0f0;"|'''Final [ADA] (U/mL)'''
 
| align="center" style="background:#f0f0f0;"|'''[Adenosine Stock Solution] (uM)'''
 
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution Added (mL)'''
 
| align="center" style="background:#f0f0f0;"|'''Final [Adenosine] (uM)'''
 
| align="center" style="background:#f0f0f0;"|'''Volume 0.05 M Phosphate Buffer Added (mL)'''
 
| align="center" style="background:#f0f0f0;"|'''Volume Inhibitor Stock solution added'''
 
| align="center" style="background:#f0f0f0;"|'''[Inhibitor Stock Solution] (uM)'''
 
| align="center" style="background:#f0f0f0;"|'''[Final Inhibitor] (uM)'''
 
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
 
|-
 
| 0.5||0.02||0.00333||52.63157895||0.6||10.52631579||1.78||0.6||65||13||3
 
|-
 
| 0.5||0.02||0.00333||61.72839506||0.6||12.34567901||1.78||0.6||65||13||3
 
|-
 
| 0.5||0.02||0.00333||74.62686567||0.6||14.92537313||1.78||0.6||65||13||3
 
|-
 
| 0.5||0.02||0.00333||94.33962264||0.6||18.86792453||1.78||0.6||65||13||3
 
|-
 
| 0.5||0.02||0.00333||128.2051282||0.6||25.64102564||1.78||0.6||65||13||3
 
|-
 
| 0.5||0.02||0.00333||200||0.6||40||1.78||0.6||65||13||3
 
|-
 
|
 
|}
 
  
==Data==
 
* Add data and results here...
 
  
 
==Notes==
 
==Notes==

Revision as of 13:56, 8 May 2013

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