User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/13

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Entry title

Objective

Use data collected yesterday to calculate Average values of the Assay and calculate Km


Data

  • All Graphs and Tables are for the Combined Data:
Final Concentration Adenosine (uM) Average velocity over 180 seconds (nm/sec) 1/[Adenosine] (1/uM) 1/average velocity (sec/nm)
200 0.000180385 0.005 5543.691027
150 0.000183794 0.006666667 5440.881764
100 0.000162678 0.01 6147.11077
40 0.000111981 0.025 8930.093256
16 7.95434E-05 0.0625 12571.75005
6.4 3.42451E-05 0.15625 29201.27683


  • Km calculations
Equation Value calculated
(-1)/Km 0.028053516
Km 35.64615572
1/Vmax 4440.372695
Vmax 0.000225206

Notes

  • For run #2 concentration 40 uM was removed as an outlier
  • For run #1 no values for concentration 150 uM were used
  • Ki values for the inhibitors were found in a paper from Planta Medica:
Flavanoid Ki value)
Myricetin 32 uM
Kaempferol 32 uM
Quericetin 26 uM


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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