User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/13

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Entry title

Objective

Use data collected yesterday to calculate Average values of the Assay and calculate Km


Data

  • All Graphs and Tables are for the Combined Data:
Final Concentration Adenosine (uM) Average velocity over 180 seconds (nm/sec) 1/[Adenosine] (1/uM) 1/average velocity (sec/nm)
200 0.000180385 0.005 5543.691027
150 0.000183794 0.006666667 5440.881764
100 0.000162678 0.01 6147.11077
40 0.000111981 0.025 8930.093256
16 7.95434E-05 0.0625 12571.75005
6.4 3.42451E-05 0.15625 29201.27683

Combined Average Velocity over COncentration.png

COMBINATION AVERAGE LINEWEAKVER-BURKEPLOT.png


  • Km calculations
Equation Value calculated
(-1)/Km 0.028053516
Km 35.64615572
1/Vmax 4440.372695
Vmax 0.000225206

Notes

  • For run #2 concentration 40 uM was removed as an outlier
  • For run #1 no values for concentration 150 uM were used
  • Ki values for the inhibitors were found in a paper from Planta Medica:
Flavanoid Ki value)
Myricetin 32 uM
Kaempferol 32 uM
Quericetin 26 uM


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