User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/13

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Objective

Use data collected yesterday to calculate Average values of the Assay and calculate Km

Description

• Calculations using all three assays were performed:
1. Velocity by Concentration was graphed for each run
2. An average velocity of all three runs was graphed by concentration
3. A Lineweaver-Burke Plot was graphed for each run and Km was calculated
4. A Lineweaver-Burke Plot was graphed for the average of the three runs and an average Km was calculated
5. Error bars were added to the average Lineweaver-Burke Plot by calculating the standard deviation of the three runs 1/velocity values
• Dhea and Mary's first assay data was added to Mike and mine assays to produce an average for four runs
1. An Average velocity of combine data was graphed by concentration
2. A Lineweaver-Burke Plot was graphed for the average of the combined data and an average Km was calculated
3. Error bars were added to the combined data average Lineweaver-Burke plot by calculating the standard deviation of the combined data 1/velocity values

Data

• All Graphs and Tables are for the Combined Data:
 Final Concentration Adenosine (uM) Average velocity over 180 seconds (nm/sec) 1/[Adenosine] (1/uM) 1/average velocity (sec/nm) 200 0.000180385 0.005 5543.691027 150 0.000183794 0.006666667 5440.881764 100 0.000162678 0.01 6147.11077 40 0.000111981 0.025 8930.093256 16 7.95434E-05 0.0625 12571.75005 6.4 3.42451E-05 0.15625 29201.27683

• Km calculations
 Equation Value calculated (-1)/Km 0.028053516 Km 35.64615572 1/Vmax 4440.372695 Vmax 0.000225206

Notes

• For run #2 concentration 6.4 uM was removed as an outlier
• For run #1 no values for concentration 150 uM were used

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