Entry title
Objective
Use data collected yesterday to calculate Average values of the Assay and calculate Km
Description
 Calculations using all three assays were performed:
 Velocity by Concentration was graphed for each run
 An average velocity of all three runs was graphed by concentration
 A LineweaverBurke Plot was graphed for each run and Km was calculated
 A LineweaverBurke Plot was graphed for the average of the three runs and an average Km was calculated
 Error bars were added to the average LineweaverBurke Plot by calculating the standard deviation of the three runs 1/velocity values
 Dhea and Mary's first assay data was added to Mike and mine assays to produce an average for four runs
 An Average velocity of combine data was graphed by concentration
 A LineweaverBurke Plot was graphed for the average of the combined data and an average Km was calculated
 Error bars were added to the combined data average LineweaverBurke plot by calculating the standard deviation of the combined data 1/velocity values
Data
 All Graphs and Tables are for the Combined Data:
Final Concentration Adenosine (uM)

Average velocity over 180 seconds (nm/sec)

1/[Adenosine] (1/uM)

1/average velocity (sec/nm)

200 
0.000180385 
0.005 
5543.691027

150 
0.000183794 
0.006666667 
5440.881764

100 
0.000162678 
0.01 
6147.11077

40 
0.000111981 
0.025 
8930.093256

16 
7.95434E05 
0.0625 
12571.75005

6.4 
3.42451E05 
0.15625 
29201.27683

Equation

Value calculated

(1)/Km 
0.028053516

Km 
35.64615572

1/Vmax 
4440.372695

Vmax 
0.000225206

Notes
 For run #2 concentration 6.4 uM was removed as an outlier
 For run #1 no values for concentration 150 uM were used
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
