Difference between revisions of "User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/12"

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(Description)
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==Description==
 
==Description==
 
*Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
 
*Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
*The original Adenosine stock solution was diluted using the table below:
+
*5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
*The assay components were mixed using the table below:
+
**150 uM adenosine stock solution was used; (stock) 150uL 5mM stock + 850 uL buffer =750 uM ->use .6mL new stock for ADA assay
 +
**200 uM adenosine stock solution was used: (stock) 200 uL 5mM stock+800 uL buffer = 1000uM ->use 0.6mL new stock for ADA assay
 +
**This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.
 +
***{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''[Old Adenosine Stock Solution] (uM)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution added (uL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Phosphate Buffer added (uL)'''
 +
| align="center" style="background:#f0f0f0;"|'''[New Adenosine Stock Solution] (uL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume New Adenosine Stock Solution (mL)'''
 +
|-
 +
| 5000||200||800||1000||1
 +
|-
 +
| 5000||150||850||750||1
 +
|-
 +
| 5000||100||900||500||1
 +
|-
 +
| 5000||40||960||200||1
 +
|-
 +
| 5000||16||984||80||1
 +
|-
 +
| 5000||6.4||993.6||32||1
 +
|}
 +
**This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.
 +
***[[Image:Screen_Shot_2013-02-05_at_9.01.33_PM.png]]
  
 
==Data==
 
==Data==

Revision as of 09:17, 15 February 2013

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Objective

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Description

  • Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
  • 5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
    • 150 uM adenosine stock solution was used; (stock) 150uL 5mM stock + 850 uL buffer =750 uM ->use .6mL new stock for ADA assay
    • 200 uM adenosine stock solution was used: (stock) 200 uL 5mM stock+800 uL buffer = 1000uM ->use 0.6mL new stock for ADA assay
    • This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.
      • {| border="1" cellpadding="4" cellspacing="0" style="border:#c9c9c9 1px solid; margin: 1em 1em 1em 0; border-collapse: collapse;"
[Old Adenosine Stock Solution] (uM) Volume Adenosine Stock Solution added (uL) Volume Adenosine Phosphate Buffer added (uL) [New Adenosine Stock Solution] (uL) Volume New Adenosine Stock Solution (mL)
5000 200 800 1000 1
5000 150 850 750 1
5000 100 900 500 1
5000 40 960 200 1
5000 16 984 80 1
5000 6.4 993.6 32 1
    • This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.
      • Screen Shot 2013-02-05 at 9.01.33 PM.png

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


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