User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/12

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Entry title

Objective

Learn how to maintain an OpenWetWare Notebook.

Description

  • Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
  • 5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
    • Dilution Tables are located below

Concentration Tables

This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.

[Old Adenosine Stock Solution] (uM) Volume Adenosine Stock Solution added (uL) Volume Adenosine Phosphate Buffer added (uL) [New Adenosine Stock Solution] (uL) Volume New Adenosine Stock Solution (mL)
5000 200 800 1000 1
5000 150 850 750 1
5000 100 900 500 1
5000 40 960 200 1
5000 16 984 80 1
5000 6.4 993.6 32 1


[ADA Stock Solution] (U/mL) Volume ADA Stock Solution Added (mL) Final [ADA] (U/mL) [Adenosine Stock Solution] (mL) Volume Adenosine Stock Solution Added (mL) Final [Adenosine] (uM) Volume 0.05 M Phosphate Buffer Added (mL) Total Volume (mL)
0.5 0.02 0.00333 1000 0.6 200 2.38 3
0.5 0.02 0.00333 750 0.6 150 2.38 3
0.5 0.02 0.00333 500 0.6 100 2.38 3
0.5 0.02 0.00333 200 0.6 40 2.38 3
0.5 0.02 0.00333 80 0.6 16 2.38 3
0.5 0.02 0.00333 32 0.6 6.4 2.38 3

Notes

This area is for any observations or conclusions that you would like to note.


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