Difference between revisions of "User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/12"

From OpenWetWare
Jump to: navigation, search
(Objective)
(fix raw html notebook nav)
 
(4 intermediate revisions by one other user not shown)
Line 2: Line 2:
 
|-
 
|-
 
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
 
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
+
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
|-
 
|-
 
| colspan="2"|
 
| colspan="2"|
Line 10: Line 10:
  
  
==Objective==
 
Repeat ADA Assay 2 times for additional data
 
  
==Description==
 
*Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
 
*5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
 
**Dilution Tables are located below
 
  
==Concentration Tables==
 
This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.
 
  
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''[Old Adenosine Stock Solution] (uM)'''
 
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution added (uL)'''
 
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Phosphate Buffer added (uL)'''
 
| align="center" style="background:#f0f0f0;"|'''[New Adenosine Stock Solution] (uL)'''
 
| align="center" style="background:#f0f0f0;"|'''Volume New Adenosine Stock Solution (mL)'''
 
|-
 
| 5000||200||800||1000||1
 
|-
 
| 5000||150||850||750||1
 
|-
 
| 5000||100||900||500||1
 
|-
 
| 5000||40||960||200||1
 
|-
 
| 5000||16||984||80||1
 
|-
 
| 5000||6.4||993.6||32||1
 
|}
 
 
This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.
 
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''[ADA Stock Solution] (U/mL)'''
 
| align="center" style="background:#f0f0f0;"|'''Volume ADA Stock Solution Added (mL)'''
 
| align="center" style="background:#f0f0f0;"|'''Final [ADA] (U/mL)'''
 
| align="center" style="background:#f0f0f0;"|'''[Adenosine Stock Solution] (mL)'''
 
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution Added (mL)'''
 
| align="center" style="background:#f0f0f0;"|'''Final [Adenosine] (uM)'''
 
| align="center" style="background:#f0f0f0;"|'''Volume 0.05 M Phosphate Buffer Added (mL)'''
 
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
 
|-
 
| 0.5||0.02||0.00333||1000||0.6||200||2.38||3
 
|-
 
| 0.5||0.02||0.00333||750||0.6||150||2.38||3
 
|-
 
| 0.5||0.02||0.00333||500||0.6||100||2.38||3
 
|-
 
| 0.5||0.02||0.00333||200||0.6||40||2.38||3
 
|-
 
| 0.5||0.02||0.00333||80||0.6||16||2.38||3
 
|-
 
| 0.5||0.02||0.00333||32||0.6||6.4||2.38||3
 
|}
 
 
==Data==
 
Run#1
 
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''Concentration of Adenosine (uM)'''
 
| align="center" style="background:#f0f0f0;"|'''Average Velocity over 180s (nm/sec)'''
 
|-
 
| 200||0.000116022
 
|-
 
| 150||0.000192818
 
|-
 
| 100||0.000139227
 
|-
 
| 40||5.55556E-06
 
|-
 
| 16||6.68508E-05
 
|-
 
| 6.4||0.000039
 
|}
 
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''1/Concentration of Adenosine (uM)'''
 
| align="center" style="background:#f0f0f0;"|'''1/Velocity (nm/sec)'''
 
|-
 
| 0.005||8619.047619
 
|-
 
| 0.006666667||5186.246418
 
|-
 
| 0.01||7182.539683
 
|-
 
| 0.025||180000
 
|-
 
| 0.0625||14958.67769
 
|-
 
| 0.15625||25641.02564
 
|}
 
  
  
Run#2
 
  
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''Concentration of Adenosine (uM)'''
 
| align="center" style="background:#f0f0f0;"|'''Average Velocity over 180s (nm/sec)'''
 
|-
 
| 200||0.00021989
 
|-
 
| 150||0.000147514
 
|-
 
| 100||0.000144751
 
|-
 
| 40||0.00010884
 
|-
 
| 16||8.12155E-05
 
|-
 
| 6.4||3.8674E-05
 
|}
 
  
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''1/Concentration of Adenosine (uM)'''
 
| align="center" style="background:#f0f0f0;"|'''1/Velocity (nm/sec)'''
 
|-
 
| 0.005||4547.738693
 
|-
 
| 0.006666667||6779.026217
 
|-
 
| 0.01||6908.396947
 
|-
 
| 0.025||9187.817259
 
|-
 
| 0.0625||12312.92517
 
|-
 
| 0.15625||25857.14286
 
|}
 
  
 
==Notes==
 
==Notes==

Latest revision as of 22:27, 26 September 2017

BDLlogo notext lr.png Biomaterials Design Lab Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Entry title

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.