Difference between revisions of "User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/12"

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(Concentration Tables)
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==Objective==
 
Learn how to maintain an OpenWetWare Notebook.
 
  
==Description==
 
*Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
 
*5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
 
**Dilution Tables are located below
 
  
==Concentration Tables==
 
This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.
 
  
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''[Old Adenosine Stock Solution] (uM)'''
 
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution added (uL)'''
 
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Phosphate Buffer added (uL)'''
 
| align="center" style="background:#f0f0f0;"|'''[New Adenosine Stock Solution] (uL)'''
 
| align="center" style="background:#f0f0f0;"|'''Volume New Adenosine Stock Solution (mL)'''
 
|-
 
| 5000||200||800||1000||1
 
|-
 
| 5000||150||850||750||1
 
|-
 
| 5000||100||900||500||1
 
|-
 
| 5000||40||960||200||1
 
|-
 
| 5000||16||984||80||1
 
|-
 
| 5000||6.4||993.6||32||1
 
|}
 
  
  
{| {{table}}
+
 
| align="center" style="background:#f0f0f0;"|'''[ADA Stock Solution] (U/mL)'''
+
 
| align="center" style="background:#f0f0f0;"|'''Volume ADA Stock Solution Added (mL)'''
 
| align="center" style="background:#f0f0f0;"|'''Final [ADA] (U/mL)'''
 
| align="center" style="background:#f0f0f0;"|'''[Adenosine Stock Solution] (mL)'''
 
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution Added (mL)'''
 
| align="center" style="background:#f0f0f0;"|'''Final [Adenosine] (uM)'''
 
| align="center" style="background:#f0f0f0;"|'''Volume 0.05 M Phosphate Buffer Added (mL)'''
 
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
 
|-
 
| 0.5||0.02||0.00333||1000||0.6||200||2.38||3
 
|-
 
| 0.5||0.02||0.00333||750||0.6||150||2.38||3
 
|-
 
| 0.5||0.02||0.00333||500||0.6||100||2.38||3
 
|-
 
| 0.5||0.02||0.00333||200||0.6||40||2.38||3
 
|-
 
| 0.5||0.02||0.00333||80||0.6||16||2.38||3
 
|-
 
| 0.5||0.02||0.00333||32||0.6||6.4||2.38||3
 
|}
 
  
 
==Notes==
 
==Notes==

Latest revision as of 14:50, 8 May 2013

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