Difference between revisions of "User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/12"

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
 
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Objective==
 
Learn how to maintain an OpenWetWare Notebook.
 
  
==Description==
 
*Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
 
*5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
 
**150 uM adenosine stock solution was used; (stock) 150uL 5mM stock + 850 uL buffer =750 uM ->use .6mL new stock for ADA assay
 
**200 uM adenosine stock solution was used: (stock) 200 uL 5mM stock+800 uL buffer = 1000uM ->use 0.6mL new stock for ADA assay
 
**This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.
 
***{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''[Old Adenosine Stock Solution] (uM)'''
 
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution added (uL)'''
 
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Phosphate Buffer added (uL)'''
 
| align="center" style="background:#f0f0f0;"|'''[New Adenosine Stock Solution] (uL)'''
 
| align="center" style="background:#f0f0f0;"|'''Volume New Adenosine Stock Solution (mL)'''
 
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| 5000||200||800||1000||1
 
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| 5000||150||850||750||1
 
|-
 
| 5000||100||900||500||1
 
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| 5000||40||960||200||1
 
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| 5000||16||984||80||1
 
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| 5000||6.4||993.6||32||1
 
|}
 
**This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.
 
***[[Image:Screen_Shot_2013-02-05_at_9.01.33_PM.png]]
 
  
==Data==
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* Add data and results here...
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==Notes==
 
==Notes==

Latest revision as of 21:27, 26 September 2017

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