User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/06: Difference between revisions
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*5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted: | *5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted: | ||
**150 uM adenosine stock solution was used; (stock) 150uL 5mM stock + 850 uL buffer =750 uM ->use . | **150 uM adenosine stock solution was used; (stock) 150uL 5mM stock + 850 uL buffer =750 uM ->use .6mL new stock for ADA assay | ||
**200 uM adenosine stock solution was used: (stock) | **200 uM adenosine stock solution was used: (stock) 200 uL 5mM stock+800 uL buffer = 1000uM ->use 0.6mL new stock for ADA assay | ||
**This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions. | **This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions. | ||
***[[Image:Screen_Shot_2013-02-05_at_9.01.20_PM.png]] | ***[[Image:Screen_Shot_2013-02-05_at_9.01.20_PM.png]] |
Revision as of 13:27, 6 February 2013
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