User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/06: Difference between revisions
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*5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted: | *5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted: | ||
**150 uM adenosine stock solution was used; (stock) 150uL 5mM stock + 850 uL buffer =750 uM ->use .6mL new stock for ADA assay | |||
**200 uM adenosine stock solution was used: (stock) 200 uL 5mM stock+800 uL buffer = 1000uM ->use 0.6mL new stock for ADA assay | |||
**This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions. | **This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions. | ||
***[[Image:Screen_Shot_2013-02-05_at_9.01.20_PM.png]] | ***[[Image:Screen_Shot_2013-02-05_at_9.01.20_PM.png]] | ||
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==Data== | ==Data== | ||
* | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Concentration of Adenosine (uM)''' | |||
| align="center" style="background:#f0f0f0;"|'''Average Velocity over 30s (nm/sec)''' | |||
|- | |||
| 200||0.00021 | |||
|- | |||
| 100||0.000183333 | |||
|- | |||
| 40||0.00012 | |||
|- | |||
| 16||0.0001 | |||
|- | |||
| 6.4||0.000047 | |||
|- | |||
| 2.56||0.000033 | |||
|} | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''1/[adenosine] (1/uM)''' | |||
| align="center" style="background:#f0f0f0;"|'''1/[velocity] (sec/nm)''' | |||
|- | |||
| 0.005||4761.904762 | |||
|- | |||
| 0.01||5454.545455 | |||
|- | |||
| 0.025||8333.333333 | |||
|- | |||
| 0.0625||10000 | |||
|- | |||
| 0.15625||21276.59574 | |||
|- | |||
| 0.390625||30303.0303 | |||
|} | |||
*Lineweaver Burke Plot: | |||
[[Image:ConcentrationvsVelocity-1.png]] | |||
*Concentration Adenosine vs Velocity: | |||
[[Image:LineweaverBurkePlot-1.png]] | |||
{| {{table}} | |||
| -1/Vmax||6163.1 | |||
|- | |||
| '''Vmax'''||'''0.000162256 nm/sec''' | |||
|- | |||
| -1/Km||-0.093 | |||
|- | |||
| '''Km'''||'''10.75 uM''' | |||
|} | |||
==Notes== | ==Notes== | ||
Concentration recalculations were taken from Dhea Patel | |||
Deleted concentration 2.56 uM point from graphs | |||
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized. | Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized. |
Revision as of 09:59, 12 February 2013
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Entry titleObjectiveLearn how to maintain an OpenWetWare Notebook. Description
Data
NotesConcentration recalculations were taken from Dhea Patel Deleted concentration 2.56 uM point from graphs Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
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