User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/01/23

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Entry title

Characterization of Myricetin and Kaempferol and Identification of Other Novel Adenosine Deaminase Inhibitors: Protocol


1) FInish Proposal for experiment:

2) Outline Protocol for next class


  • Calculation: Dilution required to yield 10mL Adenosine buffer
  • Km for Adenosine = 23 uM
  • Phosphate buffer required: 300mL


  • Conversions and Measurements: Solution
  • The following table outlines the final concentrations and volumes of the solutions used in the ADA kinetics assay to be performed

ADA kinetics table.png

  • Procedure: Buffer
  1. Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)
  2. Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L. The pH of the final solution will be 7.4. This buffer can be stored for up to 1 mo at 4°C.
  3. Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)


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