User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/01/23

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Entry title

Characterization of Myricetin and Kaempferol and Identification of Other Novel Adenosine Deaminase Inhibitors: Protocol


1) FInish Proposal for experiment:

2) Outline Protocol for next class


  • Calculation: Dilution required to yield 10mL Adenosine buffer
  • Km for Adenosine = 23 uM
  • Phosphate buffer required: 300mL


  • Conversions and Measurements: Solution
  • The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed next Tuesday.

ADA kinetics table.png


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