Difference between revisions of "User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/01/23"

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
 
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==Entry title==
 
Characterization of Myricetin and Kaempferol and Identification of Other Novel Adenosine Deaminase Inhibitors: Protocol
 
  
==Objective==
 
1) FInish Proposal for experiment: https://docs.google.com/folder/d/0B_01BNMu9uSudFZCSnVncjkxQXM/edit?docId=1M2Xy9AKW7BVladIhxcBYcx8lwDc9rqNH4ISIj0xfWy8
 
  
2) Outline Protocol for next class
 
  
  
  
==Data==
 
* Conversions and Measurements: Solution
 
 
*The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed
 
 
[[Image:ADA_kinetics_table.png]]
 
 
* Procedure: Buffer
 
#Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)
 
#Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L. The pH of the final solution will be 7.4. This buffer can be stored for up to 1 mo at 4°C.
 
#Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)
 
  
 
==Notes==
 
==Notes==

Latest revision as of 22:23, 26 September 2017

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