Difference between revisions of "User:Carly M. Montanero/Notebook/CHEM-571/2014/04/02"

From OpenWetWare
Jump to: navigation, search
(Conductivity Measurement of Pure Variables at Room Temperature)
Line 12: Line 12:
 
==Procedure==
 
==Procedure==
  
===Conductivity Measurement of Pure Variables at Room Temperature===
+
===Conductivity Measurement of Pure Variables at 4°C===
 
[[Image:4.2.cod.jac.png|800px|]]
 
[[Image:4.2.cod.jac.png|800px|]]
 
 
  
 
===Atomic Absorption Preparation===
 
===Atomic Absorption Preparation===

Revision as of 09:21, 9 April 2014

BDLlogo notext lr.png Biomaterials Design Lab: Spring 2014 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

  • Run UV-Vis and AA on room temperature 30:1 lysozyme-AuNP samples from 3/26/14.
  • Run conductivity on pure variables at room temperature.

Procedure

Conductivity Measurement of Pure Variables at 4°C

4.2.cod.jac.png

Atomic Absorption Preparation

Creating the Gold Stock Solutions

  1. Add 50 μL of HAuCl4·3H2O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.
  2. Add 100 μL of HAuCl4·3H2O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.
  3. Add 150 μL of HAuCl4·3H2O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.
  4. Add 200 μL of HAuCl4·3H2O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.
  5. Add 250 μL of HAuCl4·3H2O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.


Atomic Absorption Samples

Solutions with the following Au:lysozyme ratio at room temperature were run on the AA:

  • 30:1 lysozyme-AuNP with 0.03 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine)
  • 30:1 lysozyme-AuNP with 0.06 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine)
  • 30:1 lysozyme-AuNP with 0.09 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine)


Figures

4.2.14.aa.jac.png