Difference between revisions of "User:Carly M. Montanero/Notebook/CHEM-571/2013/11/20"

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(Procedure)
(Procedure)
Line 13: Line 13:
 
# Centrifuge 1mL of the sample for 30 min at max rpm.
 
# Centrifuge 1mL of the sample for 30 min at max rpm.
 
# Take UV-Vis measurements of the samples.
 
# Take UV-Vis measurements of the samples.
 +
 +
Solutions with the following Au:protein ratio were run on the UV-Vis:
 +
:''Gold to Invertase Ratio''
 +
::*30
 +
::*50
 +
::*70
 +
::*90
 +
::*110
 +
::*130
 +
::*150
 +
::*170
 +
::*190
 +
::*210
  
  

Revision as of 10:44, 20 November 2013

BDLlogo notext lr.png Biomaterials Design Lab: Fall 2013 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

To synthesize BSA-AuNPs and run UV-Vis on the invertase-AuNP solutions created yesterday.

Procedure

Preparing Invertase-AuNP Samples for UV-Vis

  1. Centrifuge 1mL of the sample for 30 min at max rpm.
  2. Take UV-Vis measurements of the samples.

Solutions with the following Au:protein ratio were run on the UV-Vis:

Gold to Invertase Ratio
  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210


Creating the Gold Stock Solution

  1. Add 0.0562 g of HAuCl4·3H2O to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of gold stock was 2.85 mM.


Creating the BSA Stock Solution

  1. Add 0.0432 g of BSA to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of BSA stock was 21.8 mM.


Solutions with the following Au:protein ratio were synthesized:

Gold to BSA Ratio
  • 105
  • 110
  • 115
  • 120
  • 125
  • 130
  • 135
  • 140
  • 145
  • 150


  1. As seen below, the corresponding amount of protein was added to a 50 mL volumetric flask and filled with distilled water. The samples were made in small test tubes and capped with aluminum foil.
  2. The samples were heated at 80°C for 4 hours.