# Difference between revisions of "User:Carly M. Montanero/Notebook/CHEM-571/2013/11/13"

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## Objective

To run atomic absorption (AA) on our lysozyme and myoglobin and hemoglobin samples.

## Procedure

Creating the Gold Stock Solutions

1. Add 50 μL of HAuCl4·3H2O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.
2. Add 100 μL of HAuCl4·3H2O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.
3. Add 150 μL of HAuCl4·3H2O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.
4. Add 200 μL of HAuCl4·3H2O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.
5. Add 250 μL of HAuCl4·3H2O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.

Atomic Absorption Samples

Solutions with the following Au:protein ratio were run on the AA:

Gold to Hemoglobin Ratio
• 30
• 50
• 70
• 90
• 110
• 130
• 150
• 170
• 190
• 210
Gold to Lysozyme Ratio
• 5
• 10
• 15
• 20
• 25
• 30
• 35
• 40
• 45
• 50
Gold to Lysozyme Ratio
• 30
• 50
• 70
• 90
• 110
• 130
• 150
• 170
• 190
• 210
Gold to Myoglobin Ratio
• 10
• 15
• 25
• 30
• 35
• 40
• 45
• 50