Difference between revisions of "User:Carly M. Montanero/Notebook/CHEM-571/2013/11/13"

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(Entry title)
(Procedure)
Line 14: Line 14:
 
# The final concentration of gold stock was 2.57 mM.
 
# The final concentration of gold stock was 2.57 mM.
  
 +
Solutions with the following Au:protein ratio were synthesized:
  
 
+
:''Gold to Hemoglobin Ratio''
 +
::*30
 +
::*50
 +
::*70
 +
::*90
 +
::*110
 +
::*130
 +
::*150
 +
::*170
 +
::*190
 +
::*210
 +
:''Gold to Lysozyme Ratio''
 +
::*5
 +
::*10
 +
::*15
 +
::*20
 +
::*25
 +
::*30
 +
::*35
 +
::*40
 +
::*45
 +
::*50
 +
:''Gold to Lysozyme Ratio''
 +
::*30
 +
::*50
 +
::*70
 +
::*90
 +
::*110
 +
::*130
 +
::*150
 +
::*170
 +
::*190
 +
::*210
 +
:''Gold to Myoglobin Ratio''
 +
::*10
 +
::*15
 +
::*25
 +
::*30
 +
::*35
 +
::*40
 +
::*45
 +
::*50
 
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Revision as of 11:03, 13 November 2013

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Objective

To run atomic absorption (AA) on our lysozyme and myoglobin and hemoglobin samples.

Procedure

Creating the Gold Stock Solution

  1. Add 0.0506 g of HAuCl4·3H2O to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of gold stock was 2.57 mM.

Solutions with the following Au:protein ratio were synthesized:

Gold to Hemoglobin Ratio
  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210
Gold to Lysozyme Ratio
  • 5
  • 10
  • 15
  • 20
  • 25
  • 30
  • 35
  • 40
  • 45
  • 50
Gold to Lysozyme Ratio
  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210
Gold to Myoglobin Ratio
  • 10
  • 15
  • 25
  • 30
  • 35
  • 40
  • 45
  • 50