Difference between revisions of "User:Carly M. Montanero/Notebook/CHEM-571/2013/11/06"

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(Procedure)
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==Procedure==
 
==Procedure==
 
'''Creating the Gold Stock Solution'''
 
'''Creating the Gold Stock Solution'''
# Add 0.0506 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O to a 50 mL volumetric flask and fill with distilled water.
+
# Add 0.0524 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O to a 50 mL volumetric flask and fill with distilled water.
 
# The final concentration of gold stock was 2.57 mM.
 
# The final concentration of gold stock was 2.57 mM.
  
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# The final concentration of lysozyme stock was 15 μM.
 
# The final concentration of lysozyme stock was 15 μM.
  
'''Creating the Myoglobin Stock Solution'''
+
'''Creating the Hemoglobin Stock Solution'''
# Add 0.02640 g of myoglobin to a 50 mL volumetric flask and fill with distilled water.  
+
# Add 0.02640 g of hemoglobin to a 50 mL volumetric flask and fill with distilled water.  
# The final concentration of myoglobin stock was 15 μM.
+
# The final concentration of hemoglobin stock was 15 μM.
  
  

Revision as of 10:43, 6 November 2013

BDLlogo notext lr.png Biomaterials Design Lab: Fall 2013 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

To synthesize hemoglobin-AuNPs and lysozyme-AuNPs.

Procedure

Creating the Gold Stock Solution

  1. Add 0.0524 g of HAuCl4·3H2O to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of gold stock was 2.57 mM.

Creating the Lysozyme Stock Solution

  1. Add 0.010725 g of lysozyme to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of lysozyme stock was 15 μM.

Creating the Hemoglobin Stock Solution

  1. Add 0.02640 g of hemoglobin to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of hemoglobin stock was 15 μM.


Solutions with the following Au:protein ratio were synthesized:

Gold to Hemoglobin Ratio
  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210
Gold to Lysozyme Ratio
  • 5
  • 10
  • 15
  • 20
  • 25
  • 30
  • 35
  • 40
  • 45
  • 50