Difference between revisions of "User:Carly M. Montanero/Notebook/CHEM-571/2013/10/29"

Objective

Analysis and synthesis of more protein-AuNP materials. Each group will have a different task. One (set) of groups will be doing AA on their samples from last weed (HRP-AuNPs and pepsin-AuNPs) and this week (Myoglobin-AuNPs and HRP-AuNPs). Another (set) will take UV-Vis absorbance of their samples (this week and last week). The final (set) will synthesize new protein-AuNPs.

Procedure

Creating the Gold Stock Solution

1. Add 0.0506 g of HAuCl₄·3H₂O to a 50 mL volumetric flask and fill with distilled water.
2. The final concentration of gold stock was 2.57 mM.

Creating the Lysozyme Stock Solution

1. Add 0.010725 g of lysozyme to a 50 mL volumetric flask and fill with distilled water.
2. The final concentration of lysozyme stock was 15 μM.

Creating the Myoglobin Stock Solution

1. Add 0.02640 g of myoglobin to a 50 mL volumetric flask and fill with distilled water.
2. The final concentration of myoglobin stock was 15 μM.

Synthesizing Lysozyme AuNP and Myoglobin AuNP

Solutions with the following Au:protein ratio were synthesized:

Gold to Lysozyme Ratio

• 30
• 50
• 70
• 90
• 110
• 130
• 150
• 170
• 190
• 210

Gold to Myoglobin Ratio

• 10
• 15
• 25
• 30
• 35
• 40
• 45
• 50

1. As seen in Figure 1, the corresponding amount of protein was added to a 50 mL volumetric flask and filled with distilled water. The samples were made in small test tubes and capped with aluminum foil.
2. The samples were heated at 80°C for 4 hours.

Figures

• Myoglobin UV-Vis

• Lysozyme UV-Vis

Notes

• The final gold concentration in the solutions was 0.25 mM.