Difference between revisions of "User:Bohao Liu/Notebook/Research/2014/04/29"

From OpenWetWare
< User:Bohao Liu‎ | Notebook‎ | Research‎ | 2014‎ | 04
Jump to: navigation, search
(Settings)
(fix raw html notebook nav)
 
(3 intermediate revisions by one other user not shown)
Line 2: Line 2:
 
|-
 
|-
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
+
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
|-
 
|-
 
| colspan="2"|
 
| colspan="2"|
Line 26: Line 26:
  
 
*ND=2
 
*ND=2
*mCherry = 100 mS exposure
+
*mCherry = 200 mS exposure
 
*GFP = 200 mS exposure
 
*GFP = 200 mS exposure
 
*DIC
 
*DIC
 +
  
  

Latest revision as of 22:56, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

HOG Experiments : wild - type controls

Objective

  • Make sure I can do HOG experiments again
  • Confirm Wild-type values and also expand the frequencies tested
  • Confirm the protocol: specifically that waiting 30 minutes between each set of oscillations is enough to ensure that the cells return to normal
  • See if the cells are growing

Experiment

  • Cells OD @ 10:30 = .389
  • Started flow at 11 am
  • Started experiment at 11:30 am

Settings

  • 240 second half-period
  • 48 second imaging = 6 images per cycle
  • 40 minute total time = 5 complete periods
  • ND=2
  • mCherry = 200 mS exposure
  • GFP = 200 mS exposure
  • DIC


Cells

  • 1:200 from 1.5 OD is at .973 OD 24 hours later
  • 1:100 set back 16 hours is at ~ .400 OD