Difference between revisions of "User:Bohao Liu/Notebook/Research/2014/04/29"

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(Autocreate 2014/04/29 Entry for User:Bohao_Liu/Notebook/Research)
 
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==Title==
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==HOG Experiments : wild - type controls==
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 +
 
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===Objective===
 +
*Make sure I can do HOG experiments again
 +
*Confirm Wild-type values and also expand the frequencies tested
 +
*Confirm the protocol: specifically that waiting 30 minutes between each set of oscillations is enough to ensure that the cells return to normal
 +
*See if the cells are growing
 +
 
 +
===Experiment===
 +
*Cells OD  @ 10:30 = .389
 +
*Started flow at 11 am
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*Started experiment at 11:30 am
 +
 
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====Settings====
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*240 second half-period
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*48 second imaging = 6 images per cycle
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*40 minute total time = 5 complete periods
 +
 
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*ND=2
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*mCherry = 100 mS exposure
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*GFP = 200 mS exposure
 +
*DIC
  
  
===Smaller Title===
 
*Stuff
 
#Numbered stuff
 
  
 
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Revision as of 08:31, 29 April 2014

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HOG Experiments : wild - type controls

Objective

  • Make sure I can do HOG experiments again
  • Confirm Wild-type values and also expand the frequencies tested
  • Confirm the protocol: specifically that waiting 30 minutes between each set of oscillations is enough to ensure that the cells return to normal
  • See if the cells are growing

Experiment

  • Cells OD @ 10:30 = .389
  • Started flow at 11 am
  • Started experiment at 11:30 am

Settings

  • 240 second half-period
  • 48 second imaging = 6 images per cycle
  • 40 minute total time = 5 complete periods
  • ND=2
  • mCherry = 100 mS exposure
  • GFP = 200 mS exposure
  • DIC