Difference between revisions of "User:Bohao Liu/Notebook/Research/2014/04/29"

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==Title==
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==HOG Experiments : wild - type controls==
  
  
===Smaller Title===
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===Objective===
*Stuff
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*Make sure I can do HOG experiments again
#Numbered stuff
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*Confirm Wild-type values and also expand the frequencies tested
 +
*Confirm the protocol: specifically that waiting 30 minutes between each set of oscillations is enough to ensure that the cells return to normal
 +
*See if the cells are growing
 +
 
 +
===Experiment===
 +
*Cells OD  @ 10:30 = .389
 +
*Started flow at 11 am
 +
*Started experiment at 11:30 am
 +
 
 +
====Settings====
 +
*240 second half-period
 +
*48 second imaging = 6 images per cycle
 +
*40 minute total time = 5 complete periods
 +
 
 +
*ND=2
 +
*mCherry = 200 mS exposure
 +
*GFP = 200 mS exposure
 +
*DIC
 +
 
 +
 
 +
 
 +
====Cells====
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*1:200 from 1.5 OD is at .973 OD 24 hours later
 +
*1:100 set back 16 hours is at ~ .400 OD
 +
 
  
 
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Latest revision as of 22:56, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

HOG Experiments : wild - type controls

Objective

  • Make sure I can do HOG experiments again
  • Confirm Wild-type values and also expand the frequencies tested
  • Confirm the protocol: specifically that waiting 30 minutes between each set of oscillations is enough to ensure that the cells return to normal
  • See if the cells are growing

Experiment

  • Cells OD @ 10:30 = .389
  • Started flow at 11 am
  • Started experiment at 11:30 am

Settings

  • 240 second half-period
  • 48 second imaging = 6 images per cycle
  • 40 minute total time = 5 complete periods
  • ND=2
  • mCherry = 200 mS exposure
  • GFP = 200 mS exposure
  • DIC


Cells

  • 1:200 from 1.5 OD is at .973 OD 24 hours later
  • 1:100 set back 16 hours is at ~ .400 OD