Difference between revisions of "User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/09/07"

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(Autocreate 2013/09/07 Entry for User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli)
 
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=Date=
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=09/06/2013=
  
'''List title'''
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'''Confirm The Assembly'''
# List items
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{| {{table}} cellspacing="3" width=800px
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|Plasmid Type || SD-BD002<sup>1</sup> || SD-BD002 || SD-BD002|| BD002 (-) Ctrl|| BD002 (+)Ctrl || BD002 (+) Ctrl
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|-
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| Plasmid DNA || 3.0 μl || 3.0 μl || 3.0 μl || 3.0 μl || 3.0 μl || 3.0 μl
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|-
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| BsmB1  || 1.0 μl || 0.0 μl || 0.0 μl || 1.0 μl || 0.0 μl || 0.0 μl
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|-
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| Spe1 || 0.0 μl || 1.0 μl || 0.0 μl || 0.0 μl || 1.0 μl || 0.0 μl
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|-
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| 10x FastDigest buffer + green loading dye || 1.5 μl || 1.5 μl || 1.5 μl || 1.5 μl || 1.5 μl || 1.5 μl
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|-
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| dH<sub>2</sub>O || 9.5 μl || 9.5 μl || 10.5 μl || 9.5 μl || 9.5 μl || 10.5 μl
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|-
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| &nbsp; || 15.0 μl total || 15.0 μl total || 15.0 μl total || 15.0 μl total  || 15.0 μl total || 15.0 μl total
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|-
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| Incubate at 37°C for 10 minutes.
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|}
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# Make the (1%) agarose glee and add 15μl  of restricted vector in one well and 10 μl  of ladder.
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[[Image:Image:Ste-Directed-Test.jpg|thumb|350px|]]

Revision as of 11:39, 12 September 2013

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09/06/2013

Confirm The Assembly

Plasmid Type SD-BD0021 SD-BD002 SD-BD002 BD002 (-) Ctrl BD002 (+)Ctrl BD002 (+) Ctrl
Plasmid DNA 3.0 μl 3.0 μl 3.0 μl 3.0 μl 3.0 μl 3.0 μl
BsmB1 1.0 μl 0.0 μl 0.0 μl 1.0 μl 0.0 μl 0.0 μl
Spe1 0.0 μl 1.0 μl 0.0 μl 0.0 μl 1.0 μl 0.0 μl
10x FastDigest buffer + green loading dye 1.5 μl 1.5 μl 1.5 μl 1.5 μl 1.5 μl 1.5 μl
dH2O 9.5 μl 9.5 μl 10.5 μl 9.5 μl 9.5 μl 10.5 μl
  15.0 μl total 15.0 μl total 15.0 μl total 15.0 μl total 15.0 μl total 15.0 μl total
Incubate at 37°C for 10 minutes.
  1. Make the (1%) agarose glee and add 15μl of restricted vector in one well and 10 μl of ladder.