Difference between revisions of "User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/08/06"

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# Miniprep the transformed plasmids (GAL4-VP64) to test the transfection.
 
# Miniprep the transformed plasmids (GAL4-VP64) to test the transfection.
 
 
# Pick two colonies of each plate and grow in 2mL of LB broth with 100μg/mL AMP for 10 hrs.
 
# Pick two colonies of each plate and grow in 2mL of LB broth with 100μg/mL AMP for 10 hrs.
 
 
# Miniprep the plasmids with Zyppy Plasmid Miniprep Kit from Zymo
 
# Miniprep the plasmids with Zyppy Plasmid Miniprep Kit from Zymo
  

Revision as of 13:44, 8 August 2013

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08/06/2013

Miniprep Transformed Plasmids

  1. Miniprep the transformed plasmids (GAL4-VP64) to test the transfection.
  2. Pick two colonies of each plate and grow in 2mL of LB broth with 100μg/mL AMP for 10 hrs.
  3. Miniprep the plasmids with Zyppy Plasmid Miniprep Kit from Zymo

Miniprep result

Part number 260/280 ng/ul 260/280 ng/ul
KAH59 (MV5) 140.198 1.857 277.719 1.832
KAH54 (PCVN) 169.122 1.824 217.267 1.832
KAH59 (PCVN) 203.300 1.773 66.2 1.829
KAH60 (PCVN) 33.877 1.988 169.931 1.71
KAH66 (MV5) 121.409 1.814 174.819 1.822
KAH36 162.434 1.832 130.043 1.832
  • KAH59 (MV5) produce red pellet after transformed E-coli centrifugation.