Difference between revisions of "User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/08/05"

From OpenWetWare
Jump to: navigation, search
(Date)
(Transformation)
Line 10: Line 10:
  
 
=Transformation=
 
=Transformation=
 
----
 
  
 
Transformation of Gal4-VP64 parts in '''BL21-DE3'''for transactivation of Cyan flourescent reporter gene.
 
Transformation of Gal4-VP64 parts in '''BL21-DE3'''for transactivation of Cyan flourescent reporter gene.
  
'''Parts'''
+
*'''Parts'''
  
 
{| {{table}} cellspacing=''7''
 
{| {{table}} cellspacing=''7''
Line 32: Line 30:
 
|-
 
|-
 
| KAH36 || V0120 || [K : mCh : mCh] in pcDNA3.1+/VP64-NLS vector
 
| KAH36 || V0120 || [K : mCh : mCh] in pcDNA3.1+/VP64-NLS vector
 +
 +
* 1μL of plasmid and 9μL dH2O.
 +
* 40μL of BL21-DE3 competent cells.
 +
* Culture on AMP agar plates in 37°C incubator overnight.

Revision as of 15:14, 5 August 2013

Owwnotebook icon.pngPcTF Subcloning in E. coli <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Date

Transformation

Transformation of Gal4-VP64 parts in BL21-DE3for transactivation of Cyan flourescent reporter gene.

  • Parts
BioBrick Part Vector Description
KAH59 PcVN [K : Gal4 DB : mCh : mCh] + VP64-NLS
KAH60 PcVN [K : Gal4 DB : mCh] + VP64-NLS
KAH54 PcVN [[K : Gal4 DB] + VP64-NLS
KAH59 PcN (MV5) [K : Gal4 DB : mCh : mCh] + NLS
KAH66 PcN (MV5) [K : Gal4 DB : mCh : mCh : VP64] + NLS
KAH36 V0120 [K : mCh : mCh] in pcDNA3.1+/VP64-NLS vector
  • 1μL of plasmid and 9μL dH2O.
  • 40μL of BL21-DE3 competent cells.
  • Culture on AMP agar plates in 37°C incubator overnight.