Difference between revisions of "User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/06/21"

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==Media Preparation with Hygromycine==
 
==Media Preparation with Hygromycine==
  
=Transgenic Cell Maintenance=
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'''Transgenic Cell Maintenance'''
 
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Revision as of 16:25, 23 July 2013

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06/21/2013

Media Preparation with Hygromycine

Transgenic Cell Maintenance

Cell Type Genotype Medium Supplements
(required)
Maintenance
antibiotic(s)
U2OS (adherent) Flp-in T-REx, hygro-transfected McCoy's 5A 10% FBS, 1 % pen/strep 15 ug/mL blasticidin, 60 μg/mL hygromycin
U2OS (adherent) Flp-in T-REx, hygro-transfected McCoy's 5A 10% FBS, 1 % pen/strep 15 ug/mL blasticidin, 200 μg/mL hygromycin