User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/06/06: Difference between revisions
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BD002 (3:1) = 339 ng/μl | BD002 (3:1) = 339 ng/μl | ||
BD002 (4:1) = 317 ng/μl | BD002 (4:1) = 317 ng/μl | ||
KAH187 = 441.5 ng/μl | |||
FlpE = 192 ng/μl | |||
{| {{table}} cellspacing="7" width=700px | {| {{table}} cellspacing="7" width=700px | ||
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| 1-1 || BD002 (3:1) + FlpE || 1.5 + 0.5 μg || 4.42 + 2.60 μL || 12.98μL|| 2.5μL|| 7.5 μL || 570 μL | | 1-1 || BD002 (3:1) + FlpE || 1.5 + 0.5 μg || 4.42 + 2.60 μL || 12.98μL|| 2.5μL|| 7.5 μL || 570 μL | ||
|- | |- | ||
| 1-2 || BD002 ( | | 1-2 || BD002 (4:1) + FlpE || " || 4.73 + 2.60 μL || 12.67μL|| " || " || " | ||
|- | |- | ||
| 1-3 | | 1-3 (-) Ctrl || BD002 (3:1) + --- || 2 μg || 4.42 + --- μL || 15.58μL || " || " || " | ||
|- | |- | ||
| 1-4 | | 1-4 (-)Ctrl || --- + --- || 0 μg || --- + --- μL || 20.0μL|| " || " || " | ||
|- | |- | ||
|1-5 || | |1-5 || KAH187 || 20 μg || 4.53 || 15.46μL|| " || "||" | ||
|} | |} | ||
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Revision as of 13:39, 19 June 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||
06/06/2013
Minipreps 15 μL --> 37°C/ ~15 min.
1 day before transfection:
Transfections > U2OS Flp-in T-REx lines (puro/zeo/blast) + FRT-marked mammalian transfection vector (hygro)
BD002 (3:1) = 339 ng/μl BD002 (4:1) = 317 ng/μl KAH187 = 441.5 ng/μl FlpE = 192 ng/μl
Transgene expression should be detectable after 18 hours.
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