User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/06/06: Difference between revisions
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{| {{table}} cellspacing="7" width=700px | {| {{table}} cellspacing="7" width=700px | ||
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| | | Plate-Well || Plasmid || DNA || Volume|| dH2O|| Lipo || PLUS reagent || <u>Opti-MEM (total)</u> | ||
|- | |- | ||
| 1-1 || BD002 (3:1) + FlpE || 1.5 + 0.5 μg || 4.42 + 2.60 μL || 12.98μL|| | | 1-1 || BD002 (3:1) + FlpE || 1.5 + 0.5 μg || 4.42 + 2.60 μL || 12.98μL|| 2.5μL|| 7.5 μL || 570 μL | ||
|- | |- | ||
| 1-2 || BD002 (3:1) + FlpE || " || 4.42 + 2.60 μL || 12.98μL|| " | | 1-2 || BD002 (3:1) + FlpE || " || 4.42 + 2.60 μL || 12.98μL|| " || " || " | ||
|- | |- | ||
| 1-3 || BD002 (4:1) + FlpE || " || 4.73 + 2.60 μL || 12.67μL || " | | 1-3 || BD002 (4:1) + FlpE || " || 4.73 + 2.60 μL || 12.67μL || " || " || " | ||
|- | |- | ||
| 1-4 || BD002 (4:1)+ FlpE || " || 4.73 + 2.60 μL || 12.67μL|| " | | 1-4 || BD002 (4:1)+ FlpE || " || 4.73 + 2.60 μL || 12.67μL|| " || " || " | ||
|- | |- | ||
|1-5 || BD002 (3:1) + --- || | |1-5 || BD002 (3:1) + --- || " || 4.42 + --- || 15.58μL|| " || "||" | ||
|- | |- | ||
| 1-6 || BD002 (4:1) + --- || | | 1-6 || BD002 (4:1) + --- || " || 4.73 + --- || 15.58μL || " || "||" | ||
|} | |} | ||
Revision as of 12:06, 6 June 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
06/06/2013
Transfections
BD002 (3:1) = 339 ng/μl BD002 (4:1) = 317 ng/μl PlpE = 192 ng/μl
Polycomb-ATF negative control lines: colony plates
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