User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/06/06: Difference between revisions
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PlpE = 192 ng/μl | PlpE = 192 ng/μl | ||
{| {{table}} cellspacing="7" width=700px | |||
{| | |||
|- | |- | ||
| <u>Plate-Well</u> || <u>Plasmid</u> || <u>DNA</u> || <u>Volume</u> || <u>Lipo</u> || <u>Opti-MEM (total)</u> | | <u>Plate-Well</u> || <u>Plasmid</u> || <u>DNA</u> || <u>Volume</u>|| dH2O || <u>Lipo</u> || <u>Opti-MEM (total)</u> | ||
|- | |- | ||
| 1-1 || BD002 (3:1) + FlpE || 1.5 + 0.5 μg || 4.42 + 2.60 μL || 4 μL || 500 μL | | 1-1 || BD002 (3:1) + FlpE || 1.5 + 0.5 μg || 4.42 + 2.60 μL || 12.98μL|| 4 μL || 500 μL | ||
|- | |- | ||
| 1-2 || BD002 (3:1) + FlpE || " || 4.42 + 2.60 μL || " || " | | 1-2 || BD002 (3:1) + FlpE || " || 4.42 + 2.60 μL || 12.98μL|| " || " | ||
|- | |- | ||
| 1-3 || BD002 (4:1) + FlpE || " || 4.73 + 2.60 μL || " || " | | 1-3 || BD002 (4:1) + FlpE || " || 4.73 + 2.60 μL || 12.67μL || " || " | ||
|- | |- | ||
| 1-4 || BD002 (4:1)+ FlpE || " || 4.73 + 2.60 μL | | 1-4 || BD002 (4:1)+ FlpE || " || 4.73 + 2.60 μL || 12.67μL|| " || " | ||
|- | |- | ||
|1-5 || BD002 (3:1) + --- | |1-5 || BD002 (3:1) + --- || || 4.42 + --- || 15.58μL|| || | ||
|- | |- | ||
|1-6 || BD002 (4:1) + --- | | 1-6 || BD002 (4:1) + --- || || 4.73 + --- || 15.58μL || || | ||
| | |} | ||
Revision as of 12:02, 6 June 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||
06/06/2013
Transfections
BD002 (3:1) = 339 ng/μl BD002 (4:1) = 317 ng/μl PlpE = 192 ng/μl
Polycomb-ATF negative control lines: colony plates
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