User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/04/18: Difference between revisions
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'''Digests''' <br> | |||
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* Ligations | * '''Ligations''' | ||
{| {{table}} cellspacing="3" <!-- Ligations table --> | {| {{table}} cellspacing="3" <!-- Ligations table --> | ||
|- bgcolor=#cfcfcf | |- bgcolor=#cfcfcf | ||
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* The incubation time for the ligation process was 15min at room temperature. | * The incubation time for the ligation process was 15min at room temperature. | ||
* Fast transformation, 15 min on ice. | * Fast transformation, 15 min on ice. | ||
Colony Test | |||
[[Image:Vector_Cut_(E-P).png |thumb|350px| Human Vector PT7CFE1-CHis, 3600 bp, Cut with EcoRI and PstI Separately.]] | |||
{| class="wikitable" width=400px | |||
| Plasmid DNA || 2.0 μl* | |||
|- | |||
| EcoR1 || 1.0 μl | |||
|- | |||
| Pst1 || 1.0 μl | |||
|- | |||
| 10x FastDigest buffer + green loading dye || 1.5 μl | |||
|- | |||
| dH<sub>2</sub>O || 9.5 μl | |||
|- | |||
| || 15.0 μl total | |||
|- | |||
| Incubate at 37°C for 10 minutes. | |||
|} | |||
# Make the (1%) agarose glee and add 15μl of restricted vector in one well and 10 μl of ladder. |
Revision as of 22:52, 18 April 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
04/18/2013Building a Reporter Gene to Determine Chromatin Protein Function
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