User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/12/20: Difference between revisions

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'''Table of Components for the IVT reaction'''
'''Table of Components for the IVT reaction'''
{| border="1"
! Components !! No DNA Ctrl. (μl) !! GFP Ctrl. (μl) !! Target Protein (μl) with MV2 !! Target Protein (μl) with pT-CHis
|-
|format modifier (not displayed) | Hela Lysate ||43125
|-
|format  |Accessory Proteins ||format | 16143
|-
|format  | Reaction Mix ||format | 7978
|-
|format  |pCFE-GFP DNA (0.5μg/μL) ||format | 4124
|-
|format  | Cloned DNA (0.5 μg/μL) ||format | 2718
|-
|format | Nuclease free Water ||format|
|}

Revision as of 17:06, 20 December 2012

PcTF Subcloning in E. coli <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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12/20/2012

1-Step Human Coupled in vitro protein expression

  • 1-Step Human In Vitro Protein Expression Kits enable the translation and post-transcriptional modification of full-length proteins from mRNA or plasmid templates with yields of up to 100µg/mL per reaction.

Kit Specifications

  • The PcTF gene with two different backbones were used for the protein expression.
    • pCFE1-CHis Expression vector
    • Mv2 vector , Part:BBa_J176122

Table of Components for the IVT reaction

Components No DNA Ctrl. (μl) GFP Ctrl. (μl) Target Protein (μl) with MV2 Target Protein (μl) with pT-CHis
Hela Lysate 43125
Accessory Proteins 16143
Reaction Mix 7978
pCFE-GFP DNA (0.5μg/μL) 4124
Cloned DNA (0.5 μg/μL) 2718
Nuclease free Water
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