User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/12/20: Difference between revisions
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'''1-Step Human Coupled in vitro protein expression''' | '''1-Step Human Coupled in vitro protein expression''' | ||
* 1-Step Human In Vitro Protein Expression Kits enable the translation and post-transcriptional modification of full-length proteins from mRNA or plasmid templates with yields of up to 100µg/mL per reaction. | |||
[http://www.piercenet.com/browse.cfm?fldID=0F8AB1C8-B7C2-3CC6-6B59-9BC58F0BA912 Kit Specifications] | |||
* The PcTF gene with two different backbones were used for the protein expression. | |||
** pCFE1-CHis Expression vector | |||
** Mv2 vector , Part:BBa_J176122 | |||
'''Table of Components for the IVT reaction''' |
Revision as of 16:58, 20 December 2012
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12/20/20121-Step Human Coupled in vitro protein expression
Table of Components for the IVT reaction |