User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/12/20: Difference between revisions
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'''Table of Components for the IVT reaction''' | '''Table of Components for the IVT reaction''' | ||
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! Components !! No DNA Ctrl. (μl) !! GFP Ctrl. (μl) !! Target Protein (μl) with MV2 !! Target Protein (μl) with pT-CHis | ! Components !! No DNA Ctrl. (μl) !! GFP Ctrl. (μl) !! Target Protein (μl) with MV2 !! Target Protein (μl) with pT-CHis | ||
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* The software will deduce the control data from the sample data | * The software will deduce the control data from the sample data | ||
'''RFP result''': | |||
<div class="floatright">[[Image:RFP-In_vitro-PcTF_Expression.png ]]<br>Human in-vitro Protein Expression | |||
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! Reaction !! RFP (AU) | |||
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| No DNA Negative Control|| 109 | |||
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| GFP Positive Control || 91 | |||
|- | |||
| Target Protein pC-CHis (Frozen) || 3395 | |||
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| Target Protein pC-CHis (Fresh) || 8819 | |||
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| Target Protein Mv2 || 0 | |||
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Revision as of 20:30, 20 December 2012
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||
12/20/20121-Step Human Coupled in vitro protein expression
Table of Components for the IVT reaction
Fluorescent plate reader
RFP result:
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