Difference between revisions of "User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/04/05"

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(Autocreate 2012/04/05 Entry for User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli)
 
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=Date=
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=5/4/2012=
  
'''List title'''
+
'''Culturing the colonies grew on agar plates on 4/4/2012'''
# List items
+
# BBa_I712074
 +
# BBa_I719005
 +
# BBa_B0030
 +
# BD111
 +
 
 +
'''Grow liquid cultures'''
 +
# Label 15 ml sterile culture tube(s) appropriately. Fill each tube with 2 ml of LB growth medium + appropriate antibiotic (e.g., 100 μg/ml ampicillin).
 +
# Using a sterile pipette tip, touch the bacterial streak (or pick up a single colony) and put the tip into the LB medium (bacterial end down).
 +
# Grow the cultures for 7 hours in a shaking 37°C incubator.<br><br>

Revision as of 11:40, 5 April 2012

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5/4/2012

Culturing the colonies grew on agar plates on 4/4/2012

  1. BBa_I712074
  2. BBa_I719005
  3. BBa_B0030
  4. BD111

Grow liquid cultures

  1. Label 15 ml sterile culture tube(s) appropriately. Fill each tube with 2 ml of LB growth medium + appropriate antibiotic (e.g., 100 μg/ml ampicillin).
  2. Using a sterile pipette tip, touch the bacterial streak (or pick up a single colony) and put the tip into the LB medium (bacterial end down).
  3. Grow the cultures for 7 hours in a shaking 37°C incubator.