Difference between revisions of "User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli"

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< [http://openwetware.org/wiki/Haynes_Lab Haynes Lab OWW Home]
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==04/04/11==
 
<!-- Precede finished items with a checkmark &#x2713; -->
 
* &#x2713; Assemblies: BD111, BD112, BD113
 
----
 
'''Assemblies'''
 
# BD111: KAH109/(X/P)/1123 + BBa_B0030/(S/P)/15
 
# BD112: BD111/(X/P)/1138 + BBa_I712074/(S/P)46
 
# BD113: BD111/(X/P)/1138 + BBa_I719005/(S/P)23
 
  
  
> Digests (Fermentas FD)<br>
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==Behzad's Notebook==
  
{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table -->
+
'''Project Description'''
|-valign="top"
+
* Project description coming soon
| <u>Reagent</u> || <u>Volume</u> || &nbsp;
 
| rowspan="9" |
 
|-
 
| DNA (plasmid) || up to 20 μL
 
|-
 
| 10x buffer || 3.0
 
|-
 
| enzyme 1 || 1.0
 
|-
 
| enzyme 2 || 1.0
 
|-
 
| dH<sub>2</sub>O || ---
 
|-
 
| &nbsp; || 30 μL --> 37°C/ ~30 min.
 
|}
 
{| class="wikitable" width=400px
 
| Plasmid DNA || 15.0 μl*
 
|-
 
| Fermentas FastDigest enzyme 1 || 1.0 μl
 
|-
 
| Fermentas FastDigest enzyme 2 || 1.0 μl
 
|-
 
| 10x FastDigest buffer + green loading dye || 3.0 μl
 
|-
 
| dH<sub>2</sub>O || 10.0 μl
 
|-
 
| &nbsp; || 30.0 μl total
 
|-
 
| colspan="2" | *For low yield DNA, use up to 25 μL; decrease dH<sub>2</sub>O accordingly.<br>Mix the reaction(s) thoroughly by flicking the tube.<br>Incubate at 37°C for 10 minutes.
 
|}
 
__NOTOC__
 

Revision as of 06:00, 3 September 2013

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Behzad's Notebook

Project Description

  • Project description coming soon