Difference between revisions of "User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli"

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< [http://openwetware.org/wiki/Haynes_Lab Haynes Lab OWW Home]
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----
 
'''Assemblies'''
 
# KAH213: (4) KAH54/(S/P)/430 + (1) KAH210/(X/P)/2398
 
# KAH214: " + (2) KAH211/(X/P)/1321
 
# KAH215: " + (3) KAH212/(X/P)/1621
 
# KAH216: hPCD/(S/P)/186 &#x2713; + KAH210/(X/P)/2398
 
# KAH217: " + KAH211/(X/P)/1321
 
# KAH218: " + KAH212/(X/P)/1621
 
# KAH207/V0200: KAH207/(N/S)/1836 &#x2713; + V0200/(N/S dp)/4442
 
# KAH208/V0200: KAH208/(N/S)/1884 &#x2713; + "
 
# KAH209/V0200: KAH209/(N/S)/1932 &#x2713; + "
 
 
  
> Digests (Fermentas FD)<br>
 
  
{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table -->
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==Behzad's Notebook==
|-valign="top"
 
| <u>Reagent</u> || <u>Volume</u> || &nbsp;
 
| rowspan="9" | [[Image:KAH_071211_gel1.tif|200px|Cloning digests 7/12/11]]<br>30 μL/lane, 1% agarose -->
 
|-
 
| DNA (plasmid) || up to 25 μL
 
|-
 
| 10x buffer || 3.0
 
|-
 
| enzyme 1 || 1.0
 
|-
 
| enzyme 2 || 1.0
 
|-
 
| dH<sub>2</sub>O || ---
 
|-
 
| &nbsp; || 30 μL --> 37°C/ ~30 min.
 
|}
 
  
__NOTOC__
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'''Project Description'''
 +
* Project description coming soon

Revision as of 07:00, 3 September 2013

< Haynes Lab OWW Home

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Behzad's Notebook

Project Description

  • Project description coming soon