Difference between revisions of "User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli"

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==04/04/11==
 
==04/04/11==
 
<!-- Precede finished items with a checkmark &#x2713; -->
 
<!-- Precede finished items with a checkmark &#x2713; -->
* &#x2713; Assemblies: BD111, BD112
+
* &#x2713; Assemblies: BD111, BD112, BD113
 
----
 
----
 
'''Assemblies'''
 
'''Assemblies'''
# KAH213: (4) KAH54/(S/P)/430 + (1) KAH210/(X/P)/2398
+
# BD111: KAH109/(X/P)/1123 + BBa_B0030/(S/P)/15
# KAH214: " + (2) KAH211/(X/P)/1321
+
# BD112: BD111/(X/P)/1138 + BBa_I712074/(S/P)46
# KAH215: " + (3) KAH212/(X/P)/1621
+
# BD113: BD111/(X/P)/1138 + BBa_I719005/(S/P)23
# KAH216: hPCD/(S/P)/186 &#x2713; + KAH210/(X/P)/2398
 
# KAH217: " + KAH211/(X/P)/1321
 
# KAH218: " + KAH212/(X/P)/1621
 
# KAH207/V0200: KAH207/(N/S)/1836 &#x2713; + V0200/(N/S dp)/4442
 
# KAH208/V0200: KAH208/(N/S)/1884 &#x2713; + "
 
# KAH209/V0200: KAH209/(N/S)/1932 &#x2713; + "
 
  
  
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| rowspan="9" | [[Image:KAH_071211_gel1.tif|200px|Cloning digests 7/12/11]]<br>30 μL/lane, 1% agarose -->
 
| rowspan="9" | [[Image:KAH_071211_gel1.tif|200px|Cloning digests 7/12/11]]<br>30 μL/lane, 1% agarose -->
 
|-
 
|-
| DNA (plasmid) || up to 25 μL
+
| DNA (plasmid) || up to 20 μL
 
|-
 
|-
 
| 10x buffer || 3.0
 
| 10x buffer || 3.0

Revision as of 17:47, 3 April 2012

<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext">04/04/2012,04/05/2012,04/06/2012,04/08/2012,04/09/2012,04/11/2012,05/22/2012,05/23/2012,09/19/2012,11/16/2012,11/20/2012,12/01/2012,12/14/2012,12/18/2012,12/20/2012,01/12/2013,01/21/2013,01/24/2013,03/05/2013,04/18/2013,04/22/2013,05/06/2013,05/09/2013,06/06/2013,06/10/2013,06/11/2013,06/17/2013,06/21/2013,08/02/2013,08/04/2013,08/05/2013,08/06/2013,08/08/2013,08/09/2013,09/05/2013,09/06/2013,09/07/2013,10/10/2013,10/17/2013,10/18/2013,10/21/2013,10/25/2013,10/28/2013,10/29/2013,10/31/2013,11/01/2013,11/06/2013,11/13/2013,02/05/2014</div><div style="display:none;" id="page">User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

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04/04/11

  • ✓ Assemblies: BD111, BD112, BD113

Assemblies

  1. BD111: KAH109/(X/P)/1123 + BBa_B0030/(S/P)/15
  2. BD112: BD111/(X/P)/1138 + BBa_I712074/(S/P)46
  3. BD113: BD111/(X/P)/1138 + BBa_I719005/(S/P)23


> Digests (Fermentas FD)

Reagent Volume   File:KAH 071211 gel1.tif
30 μL/lane, 1% agarose -->
DNA (plasmid) up to 20 μL
10x buffer 3.0
enzyme 1 1.0
enzyme 2 1.0
dH2O ---
  30 μL --> 37°C/ ~30 min.