User:Barry Canton/Notebook/T7 RNAP transcription of rRNA

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Introduction

I have been transcribing rRNA with T7 RNA polymerase in E. coli. I would like to estimate how much immature rRNA transcript I am producing relative to that being made on the chromosome. I would also like to estimate how efficiently the T7-transcribed rRNA is being incorporated into active, functional ribosomes.

Milestones

  1. Specific detection of wild-type and mutant 16s rRNA
  2. Relative quantification of wild-type and mutant 16s rRNA
  3. Evaluation of efficiency of processing of mutant rRNA relative to wild-type rRNA.
  4. Write & Submit paper.

Protocols

Related protocols

Recent changes

18 October 2017

     00:09  User:David M.D. Bailey‎ (diff | hist) . . (-37). . David M.D. Bailey (talk | contribs) (Publications)
 m   00:02  Klenow Assembly Method: Seamless cloning‎ (diff | hist) . . (+157). . David M.D. Bailey (talk | contribs) (The Klenow Assembly Method for the seamless cloning of overlapping double stranded DNA fragments: Very cheap alternative to the Gibson Assembly)

17 October 2017

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