User:Azadeh Khamooshi

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I am a new member of OpenWetWare!

I am an independent researcher in Synthetic Biology.

I have a Microbiology degree where I worked with various Microorganisms such as Bacteria, Viruses, Fungi and Parasites using microscope, solid and liquid media culturing and other aseptic analytical techniques. My M.Sc is in Quantitative Genetics and my Master's project was Quantitative Genetic Loci Analysis where I used molecular biology techniques working with Molecular Markers, Restriction Enzymes, DNA Extraction and PCR as well as using statistical, mathematical and quantitative calculations to find the location of quantitative genes. Then I worked as a Research Assistant on Signal Transduction Pathways using Transposons and Restriction Enzymes to knock out genes and create mutations. I worked extensively with Agaros-gel and SDS-PAGE gel electrophoresis, PCR, Inverse-PCR, Blue-White Cloning, DNA Purification and DNA Extraction.

I then worked in business development in a pharmaceutical company interacting with top executives and demonstrating the application and function of Bioinformatics products. I was then awarded PhD scholarship at the University of Arkansas where I studied Drought Tolerance Gene Expression and Protein Purification. Then I decided to do independent research on Synthetic Biology.

I am now willing to join a synthetic biology research laboratory group and I would like to network with other scientists about opportunities and recent scientific discoveries.

There are two types of synthetic biologists. The first group uses unnatural molecules to mimic natural molecules with the goal of creating artificial life. The second group uses natural molecules and assembles them into a system that acts unnaturally. In general, the goal is to solve problems that are not easily understood through analysis and observation alone and it is only achieved by the manifestation of new models. So far, synthetic biology has produced diagnostic tools for diseases such as HIV and hepatitis viruses as well as devices from biomolecular parts with interesting functions. The term “synthetic biology” was first used on genetically engineered bacteria that were created with recombinant DNA technology which was synonymous with bioengineering. Later the term “synthetic biology” was used as a mean to redesign life which is an extension of biomimetic chemistry, where organic synthesis is used to generate artificial molecules that mimic natural molecules such as enzymes. Synthetic biologists are trying to assemble unnatural components to support Darwinian evolution. Recently, the engineering community is seeking to extract components from the biological systems to test and confirm them as building units to be reassembled in a way that can mimic the living nature. In the engineering aspect of synthetic biology, the suitable parts are the ones that can contribute independently to the whole system so that the behavior of an assembly can be predicted. DNA consists of double-stranded anti-parallel strands each having four various nucleotides assembled from bases, sugars and phosphates which are made of carbon, nitrogen, oxygen, hydrogen and phosphorus atoms. In the Watson-Crick model, A pairs with T and G pairs with C although occasionally some diversity exists. This simplification doesn’t exist in proteins. With analysis and observation alone, scientists convince themselves that the paradigms are the truth and if the data contradicts the theory, the data normally is discarded as an error, where synthesis encourages scientists to cross into the new land and define new theories. The same synthesis has long been used in chemistry such as chromatography. The combination of chemistry, biology and engineering can therefore create artificial Darwinian systems. (Benner and Sismour 2005)

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