User:Anthony Salvagno/Notebook/Research/2011/04/13/pALS PCR Troubleshooting: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(New page: {{qn}} ==Previous== The temperature in the lab has forced me to lower the temperature on the thermal cycler to 50C for annealing and that produced a PCR product. It also gave me less smear...) |
(No difference)
|
Revision as of 09:28, 13 April 2011
Quick Navigation: Pages by Category | List of All Pages | Protocols
Previous
The temperature in the lab has forced me to lower the temperature on the thermal cycler to 50C for annealing and that produced a PCR product. It also gave me less smear than I typically receive when doing this experiment so that is positive. I will now try to do PCR with labeled reverse primer and unlabeled forward primer and work my way through the tethering construct construction.
