User:Andrew K Farag/Notebook/Andrewfarag/2014/01/30: Difference between revisions
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|style="background-color: #EEE"|[[Image: | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
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== | |||
==objective== | |||
The objective for today is to begin the synthesis of BSA:L-Cysteine Nanocrystals | |||
==Preparation of stock solutions== | |||
# BSA | |||
#L-Cysteine | |||
#Sodium Hydroxide | |||
#Sodium Sulfide | |||
==Protocol BSA:L-cysteine Nanocrystals== | |||
# In test tube mix 180 uL of 5mM Zinc Nitrate and 20 uL of 5mM Manganese Acetate | |||
# Add 200 uL of 40 mM Ammonium Acetate | |||
# Add 200 uL of Protein:ligand in various ratios from 0% to 100% protein | |||
# Adjust pH to 8.2 using roughly 250 uL of 10mM Ammonium Hydroxide, and bring volume of water to 2.3 mL | |||
# Incubate at room temp for 1 hour | |||
# Add 200 uL of 5mM Sodium Sulfide drop wise while vortexing | |||
# Incubate for five days at 37 degrees Celsius | |||
==Table of BSA:L-Cysteine ratios== | |||
{| {{table}} | |||
| mol of protein||2.00E-09||1.80E-09||1.60E-09||1.40E-09||1.20E-09||1.00E-09||8.00E-10||6.00E-10||4.00E-10||2.00E-10||0 | |||
|- | |||
| percentage of protein||100||90||80||70||60||50||40||30||20||10||0 | |||
|- | |||
| percentage of ligand||0||10||20||30||40||50||60||70||80||90||100 | |||
|- | |||
| mol of ligand||0||2.00E-10||4.00E-10||6.00E-10||8.00E-10||1.00E-09||1.20E-09||1.40E-09||1.60E-09||1.80E-09||2.00E-09 | |||
|} | |||
Revision as of 09:56, 29 January 2014
 Biomaterials Design Lab
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objectiveThe objective for today is to begin the synthesis of BSA:L-Cysteine Nanocrystals Preparation of stock solutions
Protocol BSA:L-cysteine Nanocrystals
Table of BSA:L-Cysteine ratios
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