Difference between revisions of "User:Andrew K Farag/Notebook/Andrewfarag/2013/10/22"

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==Notes==
 
==Notes==
This area is for any observations or conclusions that you would like to note.
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Final volume is 5mL, and final concentration of gold is 0.25mM. Initial concentration of gold is 2.52mM.
  
  

Revision as of 09:35, 22 October 2013

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Objective

  1. Synthesize Pepsin-AuNPs for reactivity tests tomorrow
  2. Determine the Au:Protein ratio required for fiber formation for pepsin and HRP

Description

  1. Synthesis of HRP-AuNPs and Pepsin-AuNPs
    1. Into test tube that will contain a total of 5mL add
    2. Au solution such that the final concentration is 0.25mM
    3. Protein solution such that the final concentration reflects the following Au:Protein ratios
      1. 30
      2. 50
      3. 70
      4. 90
      5. 110
      6. 130
      7. 150
      8. 170
      9. 190
      10. 210
    4. Water up to 5mL

Data

Stock Solutions

  1. HRP
    1. 67.3mg in 100mL of water ---> 15.3 μM
  2. Pepsin
    1. 54.4mg in 100mL of water ---> 15.7 μM
  3. Gold
    1. Need 50mL of 2.52mM

Notes

Final volume is 5mL, and final concentration of gold is 0.25mM. Initial concentration of gold is 2.52mM.


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