User:Andrew K Farag/Notebook/Andrewfarag/2013/10/09: Difference between revisions

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<br> Absorbance at 249nm=Molar absortivity of Adenosine at 249nm * concentration of Adenosine + molar absortivity of Inosine at 249 * concentration of Inosine
<br> Absorbance at 249nm=Molar absortivity of Adenosine at 249nm * concentration of Adenosine + molar absortivity of Inosine at 249 * concentration of Inosine
<br> Absorbance at 259nm=Molar absortivity of Adenosine at 259nm * concentration of Adenosine + molar absortivity of Inosine at 259nm * concentration of Inosine
<br> Absorbance at 259nm=Molar absortivity of Adenosine at 259nm * concentration of Adenosine + molar absortivity of Inosine at 259nm * concentration of Inosine
<br> Molar absortivities are calculated from the the first lab in september's data.
<br> Molar absortivity of Inosine at 249 nm is 1107 M<sup>-1</sup>cm<sup>-1</sup>.
<br> Molar absortivity of Inosine at 259 nm is 6380 M<sup>-1</sup>cm<sup>-1</sup>.
<br> Molar absortivity of Adenosine at 249 nm is 14500 M<sup>-1</sup>cm<sup>-1</sup>.
<br> Molar absortivity of Inosine at 249 nm is 13280 M<sup>-1</sup>cm<sup>-1</sup>.
<br>[[Image:Concwithinhibitor.png]]


==Data==
==Data==

Revision as of 13:09, 14 October 2013

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Objective

We are running the same procedure as yesterday only with an inhibitor, EHNA.

Description

The absorbance graph is saved every 15 seconds for a period of 10 minutes total. Using the following equations, concentrations of Adenosine and Inosine with the inhibitor are calculated.
Absorbance at 249nm=Molar absortivity of Adenosine at 249nm * concentration of Adenosine + molar absortivity of Inosine at 249 * concentration of Inosine
Absorbance at 259nm=Molar absortivity of Adenosine at 259nm * concentration of Adenosine + molar absortivity of Inosine at 259nm * concentration of Inosine
Molar absortivities are calculated from the the first lab in september's data.
Molar absortivity of Inosine at 249 nm is 1107 M-1cm-1.
Molar absortivity of Inosine at 259 nm is 6380 M-1cm-1.
Molar absortivity of Adenosine at 249 nm is 14500 M-1cm-1.
Molar absortivity of Inosine at 249 nm is 13280 M-1cm-1.

Data

EHNA stock

  1. 5mg EHNA in 1mL DMSO ---> 15.9mM EHNA
  2. (1.9μL)(15.9mM EHNA)=(10mL)(C2). C2 ---> 3μM EHNA

The reaction samples will contain roughly 1nM EHNA

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




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